Title: Associate Professor, Department of Human and Molecular Genetics
Office: AHC1 418B
Department(s): Human and Molecular Genetics
Dr Tempest took her undergraduate work at Brunel University (London), earning her BSc in Applied Biology. During this time she established the research laboratory of Professor Darren Griffin. Her PhD thesis in that laboratory was "The association between sperm aneuploidy and male infertility: screening, aetiology and possible routes to alternative therapy".
Dr Tempest's expertise focuses on the chromosomal aspects of infertility and toxicant in humans utilizing new cytogenetic and molecular technologies. Remaining at Brunel University after her PhD, she first developed an infertility screening kit for rapid identification of porcine chromosomal rearrangements. Following this, she moved to the University of Kent (Canterbury) to develop avian chromosome paints and conduct comparative mapping projects. There she became interested in combining nano-technology with conventional cytogenetics, using quantum dots as fluorescent in situ hybridization (FISH) probes. In 2006, she joined the laboratory of Professor Renée Martin, at the University of Calgary. At Calgary, Dr. Tempest investigated the effect of chemotherapy on sperm aneuploidy, intra- and inter-individual variations in sperm aneuploidy and chromosome pairing and recombination during meiosis. During 2008-2009 she devoted efforts to preimplantation genetic diagnosis on single cells (blastomeres), working at the London Bridge Centre for Infertility, Genetics and Gynaecology (London, UK) with Dr. Alan Handyside, a pioneer who achieved the world's first human PGD diagnosis.
Dr Tempest's research has resulted in publications in the top reproduction and cytogenetic journals, including Nature for her contribution to the chicken genome project at the University of Kent. She was recipient of 2006 and 2007 Petro Canada Young Innovators Awards and the 2006 Champion Technologies Award, and has successfully obtained research funding from the Canadian Institutes of Health Research. Dr Tempest has been involved in the organization of international conferences.
At FIU Dr. Tempest will establish a reference reproductive genetics "gold standard" laboratory that will be a pivotal component of joint efforts between the College of Medicine and College of Engineering to develop novel, robust, and sensitive methods for monitoring exposure to chemical toxins.
- Tempest HG and Simpson JL (In press). Role of preimplantation genetic diagnosis in current infertility practice. International Journal of Infertility and Fetal Medicine
- Tempest HG. (In press). Meiotic Recombination Errors, the Origin of Sperm Aneuploidy and Clinical Recommendations. Systems Biology in Reproductive Medicine
- Tempest HG, Cheng SY, Gillot D, Thornhill AR, Handyside AH, Griffin DK. Automated spot-counting for chromosome abnormalities in sperm - a suitable alternative to manual scoring? Asian Journal of Andrology 12:257-262 (2010).
- Skinner BM, Robertson LB, Tempest HG, Langley EJ, Ioannou D, Fowler KE, Crooijmans RP, Hall AD, Griffin DK, Volker M Comparative genomics in chicken and Pekin duck using FISH mapping and microarray analysis. BMC Genomics 10:357 (2009).
- Tempest HG and Martin RH. Cytogenetic risks in chromosomally normal infertile men. Current Opinions in Obstetrics and Gynaecology 21:223-7 (2009).
- Ioannou D*, Tempest HG*, Ellis M, Thornhill AR, Griffin DK Quantum Dots as new-generation fluorochromes for FISH: An appraisal. Chromosome Research 17:519-530 (2009). *Joint first authors
- Skinner BM, Robertson LB, Tempest HG, Langley EJ, Ioannou D, Fowler KE, Crooijmans RP, Hall AD, Griffin DK, Volker M. Comparative genomics in chicken and Pekin duck using FISH mapping and microarray analysis. BMC Genomics 10:357 (2009).
- Tempest HG, Ko E, Chan P, Robaire B, Rademaker A, Martin RH. Intra-individual variations in sperm aneuploidy frequencies in control donors. Fertility and Sterility 91:185-192 (2009).
- Tempest HG, Homa ST, Routledge EJ, Garner A, Zhai XP, Griffin DK Plants used in Chinese medicine for the treatment of male infertility possess antioxidant and anti-oestrogenic activity. Systems Biology in Reproductive Medicine 54:185-195 (2008).
- Griffin DK, Robertson LB, Tempest HG, Vignal A, Fillon V, Crooijmans RPMA, Groenen MAM, Deryusheva S, Gaginskaya E, Carré W, Waddington D, Talbot R, Völker M, Masabanda JS, Burt DW. Whole genome comparative studies between chicken and turkey and their implications for avian genome evolution. BMC Genomics 9:168 (2008).
- Tempest HG, Ko E, Chan P, Robaire B, Rademaker A, Martin RH. Sperm aneuploidy frequencies analysed before and after chemotherapy in testicular cancer and Hodgkin's lymphoma patients. Human Reproduction 23: 251-258 (2008).
- Nicopoullos JDM, Gilling-Smith C, Almeida PA, Homa S, Nice L, Tempest HG, Ramsay JWA. The role of sperm aneuploidy as a predictor of the success of intracytoplasmic sperm injection? Human Reproduction 23: 240-250 (2008).
- International Chicken Genome Sequence Consortium. (Multi-author including Tempest HG. Sequence and comparative analysis of the chicken genome provide unique perspectives on vertebrate evolution. Nature 432: 695-716 (2004).