Dr. Raymond joined Herbert Wertheim College of Medicine in 2012. Her research has focused on understanding the impact and role of extracellular vesicles (EVs) in the pathogenesis of neurotropic viruses specifically, Human Immunodeficiency Virus (HIV) and Zika Virus(ZIKV). EVs include microvesicles and exosomes both of which have been shown to modulate immune responses, to disrupt cellular functions, and to contain several oncological biomarkers critical for some cancer diagnostics. Dr. Raymond’s lab is among the first to demonstrate a potential role for exosomal EVs in HIV-associated neuropathogenesis. Her research group has shown in vitro that HIV-infected cells release EVs containing the HIV Nef protein and has demonstrated ex vivo the presence Nef-containing EVs in the serum of aviremic HIV-infected subjects. However, the functional role of these Nef-containing EVs in HIV neuropathogenesis is still unknown. Dr. Raymond seeks to understand how the content (and function) of brain cell-derived EVs vary upon HIV infection and/or opiate exposure. Ultimately, the Raymond lab research focuses on the identification of EV-based associated with biomarkers HIV neuropathogenesis and opiate addiction.
Additional Research Interests:
- Developing EV-based nanocarriers for delivery of antiviral drugs to the Central Nervous System.
- Investigating the impact of EVs on antiviral immune response mechanisms.
- Utilizing NanoString Technology to identify cellular and EV factors associated with neuropathogenic ZIKV infection.
Education and Training
- PhD, 2005 - Microbiology and Immunology, Kratz School of Medicine at Temple University, Philadelphia, PA
- Master of Science, Biology, 1999 New York University, New York, NY
- BS, 1991 Massachusetts Institute of Technology, Cambridge, MA
- Post-Doctoral Fellowships:
(1) Wistar Institute HIV/AIDS Group, University of Pennsylvania School of Medicine Philadelphia, PA
(2) Morehouse School of Medicine, Department of Microbiology & Immunology, Atlanta, GA
- Microbiology and Immunology - antiviral innate immunity, macrophage/monocyte biology
- Virology/Neurovirology– HIV/AIDS, NeuroAIDS, and HIV Nef protein in viral pathogenesis
- Substance abuse
- Raymond AD, Campbell-Sims TC, Khan M, Lang M, Huang MB, Bond VC, Powell MD. HIV Type 1 Nef is released from infected cells in CD45(+) microvesicles and is present in the plasma of HIV-infected individuals. AIDS Res Hum Retroviruses. 2011 Feb;27(2):167-78. PMID: 20964480
- Ding H, Sagar V, Agudelo M, Pilakka-Kanthikeel S, Atluri VS, Raymond A, Samikkannu T, Nair MP. Enhanced blood-brain barrier transmigration using a novel Transferrin-conjugated fluorescent magneto-liposome nanoformulation. Nanotechnology 2014 Feb 7;25(5); PMID: 24406534
- Sudheesh Pilakka-Kanthikeel, Andrea Raymond, Venkata Subba Rao Atluri, Vidya Sagar, Shailendra K Saxena and Madhavan Nair. SAMHD1 facilitated HIV restriction in astrocytes is regulated by miR-155 and 181a. Journal of Neuroinflammation. 2015, PMID:25890101
- Roy U, Ding H, Pilakka-Kanthikeel S, Raymond AD, Atluri V, Yndart A, Kaftanovskaya EM, Batrakova E, Agudelo M, Nair M. Preparation and characterization of anti-HIV nanodrug targeted to microfold cell of gut-associated lymphoid tissue. Int J Nanomedicine. 2015 Sep 18;10:5819-35. PMID: 26425084
- Raymond AD, Diaz P, Chevelon S, Agudelo M, Yndart-Arias A, Ding H, Kaushik A, Jayant RD, Nikkhah-Moshaie R, Roy U, Pilakka-Kanthikeel S, Nair MP., Microglia-derived HIV Nef+ exosomes impairment of the blood-brain-barrier is treatable by nanomedicine-based delivery of Nef peptides. J Neurovirol. 2016 Apr;22(2):129-39;.PMID:26631079.
- Khan MB, Lang MJ, Huang MB, Raymond A, Bond VC, Shiramizu B, Powell MD. Nefexosomes isolated from the plasma of individuals with HIV-associated dementia (HAD) can induce Aβ1-42 secretion in SH-SY5Y neural cells. J Neurovirol. 2016, PMID: 26407718.